Becke Line Dispersion Staining
This is a collection of glass fiber that has a
refractive index of 1.550 in a high dispersion liquid with
a refractive index of 1.560. These are the colors shown as the distance between the
objects on the slide and the objective is increased.
Transmitted Brightfield Illumination With the Substage Iris Closed Down
Definition/Function:
As the distance between the object and the objective is increased the Becke line moves
in the direction of the highest refractive index.
If the mounting medium has a higher refractive index than the particle then the bright
Becke line moves into the mounting medium. If the
particle has the higher refractive index then the bright Becke Line moves into the
particle. If the refractive index of the particle and
the mounting medium match at some visible wavelength but the dispersion curve of the
mounting medium has a greater slope than that of the
particle then the wavelength for which the particle has the higher refractive index move
into the particle and the wavelengths for which
the mounting medium has the higher refractive index move into the medium. These two
Becke Lines are colored.
Significance in the Environment:
Characteristic Features:
Associated Particles:
References:
