Apparent Birefringence
There is an air bubble trapped between these two glass
fibers that are bound together at their
terminations. The high refractive index difference between the air and the glass
results in much of the light being
reflected at the interface. When viewed between crossed polars the small amount of
light that is transmitted becomes
visible against the dark background. If one of the polarizing filters is rotated the
small amount of light transmitted
appears dark relative to the much greater intensity of light coming through the
adjacent field of view.
Transmitted Off Crossed Linear Polarized Light
Definition/Function:
Apparent birefringence is the result of a change in the polarization of light from the
polarizing filter in the base of
the microscope (the polarizer) by the specimen on the stage that is not the result of
birefringence in the specimen. There are two
primary conditions that result in apparent birefringence. The first is reflection due to
differences in the refractive
index of the mounting medium and the specimen. Glass fiber mounted in a medium with a
very different refractive index
will reflect light preferentially that is polarized parallel to the length of the fiber.
The light that transmits at the
edge of the fiber will be preferentially polarized perpendicular to the length of the
fiber. The result is a rotation of
the polarization of a portion of the transmitted beam. A portion of this rotated beam
will pass through the polarizing
filter above the objective (the analyzer). This is different than the stress
birefringence that is some times present at
the edge of a glass fiber with an epoxy coating. Stress birefringence is actually due to
birefringence induced by stress.
The second cause of apparent birefringence is due to absorption of light at the edge of
a conductive material. Opaque
objects can be identified as conductive or non-conductive by the presence or absence of
light at their edge when viewed
between crossed polarizing filters.
Glass fiber is a fiber composed of a material in a "glassy" state. A "glass" is any of a
large class of materials that
solidify from a molten state without crystallization and with random molecular
orientation. They are regarded physically
as supercooled liquids rather than true solids (based on the definition in the AMERICAN
HERITAGE DICTIONARY). Chemically
they generally contain silicon along with a number of other elements though carbon-based
glasses are also common in some
environments. It is estimated that there are in excess of 50,000 different chemical
compositions for glass (MATERIALS
HANDBOOK, by George S. Brady and Henry R. Clauser). Glass is easily formed at elevated
temperature and can be made into
fibers intentionally, as an artifact of thermal forming, or as a natural process as in
the case of volcanic glass fiber
(Pele's Hair).
"Glass fiber", as identified by light microscopy, is defined by its morphology and a few
optical properties. Its
cylindrical shape (a result of surface tension at elevated temperature) is best seen by
the relief gradient at
the edge of the fiber when viewed with transmitted oblique illumination. The random
molecular structure is demonstrated by
the fact that the fiber is isotropic (disapears in all orientations when viewed between
linear polarizing filters at 90
degrees to one another). The brittle nature of the fiber is shown by the lack of plastic
deformation at the terminations
of the fiber.
Significance in the Environment:
The apparent birefringence at the edge of glass fiber must not be confused with the
presence of asbestos associated with
the glass fiber. Asbestos fibers will some times align with the glass fiber but that
alignment will not be perfectly at
the edge and it will have an abrupt termination at the end of the asbestos fiber. Some
glass fibers mounted in the 1.680
refractive index liquid used to identify amosite asbestos fibers will show some apparent
birefringes at the edge of the
fibers.
Characteristic Features:
Associated Particles:
References:
Brady, George S. and Henry R. Clauser, MATERIALS HANDBOOK, 11th Edition, ISBN
0-07-007069-5, pp. 341-350
http://www.microlabnw.com/index/Glass%20Fiber%20and%20Health%20Complaints.pdf
Parker, Sybil P. (ed), McGRAW-HILL ENCYCOPEDIA OF PHYSICS, ISBN 0-07-045253-9, p.27.